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The abundance of cultivable TFEs decreased exponentially with sediment depth with a half-life of 350 years.

This is likely because endospores are not lysed by nucleic acid extraction procedures (Wunderlin et al., 2014) and because their abundance is low relative to vegetative microbial cells.

So far, investigations of the abundance of thermophilic anaerobic endospores in cold marine sediments and estimates of their rates of supply to the seafloor only considered sulfate-reducing members of the endospore community.

We furthermore aimed to determine the time course of reactivation of the germinating TFEs.